Deteksi Kebuntingan pada Kambing Lokal Menggunakan Metode Punyakoti dengan Konsentrasi Urin dan Aquades Berbeda pada Pertumbuhan Biji Kacang Hijau

Abstract

The objective of this study was to determine whether the Punyakoti method can be used to detect
pregnancy in goats by modifying the amount of distilled water (aquadest) added to goat urine to
observe growth inhibition in mung bean seeds. This study was designed using a Completely
Randomized Design (CRD) with four treatments and six replications. The treatments involved
different concentrations of aquadest added to the urine: P1 (1:4), P2 (1:8), P3 (1:12), and P4
(1:12) using urine from non-pregnant goats. The observed variables were the germination of each
seed and the length of the sprouts on the fifth day. Data were analyzed using Analysis of Variance
(ANOVA), and if significant or highly significant differences were found, Duncan’s Multiple
Range Test (DMRT) was applied. The ANOVA results showed a significant difference in the  sprout length of mung beans due to the variation in goat urine and aquadest concentrations.
Treatment P1 (2.36 cm) produced significantly shorter sprouts (P<0.05) compared to P2 (5.75
cm), P3 (8.38 cm), and P4 (12.02 cm). The shorter sprout length in treatment P1 is likely
influenced by the higher concentration of urine diluted with aquadest. In treatment P4, mung bean
sprouts were longer, as this treatment used urine from non-pregnant goats. As a comparison,
treatment P3, which used the same urine-to-aquadest ratio as P4, showed different sprout lengths
because the urine came from pregnant goats. This difference may be due to the higher
concentration of abscisic acid (ABA) in the urine of pregnant goats. Based on the results, it can
be concluded that the Punyakoti method can be used as an alternative method for detecting
pregnancy in goats with 100% accuracy. The 1:4 concentration was the most effective, as it
showed the clearest growth inhibition compared to the 1:8 and 1:12 concentrations. This
demonstrates that different concentrations of aquadest affect the degree of growth inhibition in
mung bean seeds