Quality of Etawah Crossbreed Spermatozoa In Combination of Tris and Several Types Egg Yolks Diluent

Abstract

The aims of this study was to determine the quality of Etawah crossbreed (PE) spermatozoa
diluted with a combination of tris diluent and various types of egg yolks. The research
method employed a Completely Randomized Design (CRD) with three treatments and six
replications, consisting of (P1) 80% tris aminomethane + 20% commercial chicken egg yolk
(KTR), (P2) 80% tris aminomethane + 20% local chicken egg yolk (KTK),and (P3) 80% tris
aminomethane + 20% quail egg yolk (KTP). The observed daily qualities of PE spermatozoa
included motility and viability until the motility reached 40%. The results showed that the
motility of PE spermatozoa on day 4, P3 (40.00%) was significantly higher (P<0,05) than P1
(25,83) and P2 (30,00). The viability of PE spermatozoa showed P3 (45.47%) was
significantly higher (P<0,05) than P1 (34,65) and P2 (39,93). In conclusion that combination
of tris diluent and various types of egg yolks could maintain the quality of PE spermatozoa on
day 3 above 40%, in accordance with the minimum motility requirments for spermatozoa as
in SNI 486.3:2014, which is a minimum of 40%, suitable for use in artificial insemination
Vol. 3 No. 1, Januari 2024 ISSN: 2442-7012
Jurnal Penelitian Peternakan Lahan Basah 19
programs. The combination of tris-quail egg yolk diluent better results maintaining the
quality of Etawah crossbreed untill on day 4 of storage.